serum/plasma kit (Catalog no. 217184, Qiagen, Hilden, Germany) followed by Agencourt RNAClean XP beads (Catalog no. A63987, Beckman Coulter Inc., Indianapolis, IN, USA). Extracted RNA was then converte
final PCR product was then visualized under ultravi-olet light. Cloning and sequencing of PCR products Extraction of amplicons of PCR samples with high band intensities using a QIAquick Gel Extraction Kit (QIAGEN, Hilden, Germany) was conducted from agarose gels. The samples were then cloned into a p
Qiagen (Hilden, Germany) pÕ , power SYBR® Green PCR master mix Applied Biosystems (Foster City, CA, USA) ì' . Anti--actin, PPAR, C/EBP, SREBP, FAS, TLR2 "A TLR4 antibodies Santa Cruz Biotechnology (Santa Cruz, CA, USA) pÕ Ø' ! , Anti-pThr172-AMPK antibodies Cell Signaling Technology (Danvers, MA, USA) pÕ ì' . 2. CE
