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TruSeq Custom Amplicon v1 - Illumina, Inc.

TruSeq Custom Amplicon v1 - Illumina, Inc.

The TruSeq Custom Amplicon Library Preparation Kit supports high levels of multiplexing, while providing excellent specificity and uniformity. An example TruSeq Custom Amplicon experiment was performed by following the workflow described in Figure 1. Representative uniformity data, with percent of bases at least 0.2× the mean sequencing depth,

Position Specific Alternative Splicing and Gene Expression .

Position Specific Alternative Splicing and Gene Expression .

Screen Tape (Agilent) and a Victor3 Multilavel plate reader (PerkinElmer), respectively. 1μg of total RNA from each sample was reverse transcribed into cDNA and amplified using the Illumina TruSeq RNA Sample Prep Kit v2. For library preparation, mRNA was enriched by oligo-d

Global analysis of gene expression dynamics within the .

Global analysis of gene expression dynamics within the .

using the Agencourt RNAClean XP kit (Beckman Coulter Genomics). Then, first-strand cDNA synthesis was primed with an N6 randomized primer. After fragmentation, Illu-mina TruSeq sequencing adapters were ligated in a strand-specific manner to the 5’ and 3’ ends of the cDNA fragments,

Design and Validation of a 2nd Tier Next Generation Sequencing (NGS .

Design and Validation of a 2nd Tier Next Generation Sequencing (NGS .

TruSeq Custom Amplicon (TSCA) panel. September 13, 2017 7. Illumina MiSeq. Specification. Ion Torrent S5. 10 ng. Recommended Minimum DNA Input. 1 ng. Paired-end reads. Sequence Reads. Single end reads. Ligation capture/amplification. Library Prep/ Gene Targeting. Multiplex PCR. Reversible terminator with