C o n t en t sWarranty. iIntroduction. 1Getting Started. 1Initial Inspection. 1Battery Installation. 1Setup . . 2Keypad. 9Main Display. 11Menu Layout. 11Alpha/Numeric Entry. 12System Menu. 13Date/Time. 13GLP. 14Language. 18Item # 605596Rev DDrawing # A605596March 2009 2009 YSI Incorporated.The YSI logo and Confidence Solution are registeredtrademarks of YSI Incorporated.Teflon is a registered trademark of E. I. du Pont de Nemours and Company.Microsoft and Windows are registered trademarks of the MicrosoftCorporation in the United States and other countries.Pentium is a registered trademark of Intel Corporation.Radix Point. 18Logging. 18Auto Shutoff. 19Backlight. 19SW Version (Software Version). 19Serial #. 19Unit ID. 20Parameters: Setup, Display, Auto Stable, and Calibration. 20Temperature. 20

Dissolved Oxygen (DO). 21Sensor Maintenance. 60Barometer. 31Sensor Storage. 66Conductivity. 32pH. 38ORP. 42Ammonium, Nitrate, Chloride. 45Taking Measurements. 51Dissolved Oxygen. 51Conductivity . . 52pH/ORP. 52Ammonium, Nitrate, and Chloride. 52Troubleshooting. 68Help . 68Error Messages . 69Dissolved Oxygen. 70Restore Default Calibration Values . 70Accessories / Part Numbers. 71Declaration of Conformity. 75Logging Data. 52Recycling. 78Files and Site Lists. 53Contact Information. 79File Memory. 53Ordering and Technical Support. 79Viewing Saved Data. 53Service Information. 79Site List. 54Folder. 54Delete Data. 54Data Manager Desktop Software. 55Using the Communications Saddle. 56Manage Logged Data . 57Real Time Studies. 57Configure Instruments. 58Care, Maintenance, and Storage. 58Updating Instrument Firmware. 58General Maintenance. 58

Warran t yThis page lef t in t en t i o nally blankThe YSI Professional Plus Instrument (Pro Plus) is warranted for three (3) yearsfrom date of purchase by the end user against defects in materials and workmanship,exclusive of batteries and any damage caused by defective batteries. Pro Plus fieldcables are warranted for two (2) years from date of purchase by the end user againstdefects in material and workmanship (6 months for non-field rugged cables*). ProPlus sensors (pH, ORP, pH/ORP combo, Polarographic DO) are warranted forone (1) year from date of purchase by the end user against defects in material andworkmanship (6 months for ammonium**, nitrate**, chloride**, and Galvanic DO).Pro Plus systems (instrument, cables & sensors) are warranted for 90 days fromdate of purchase by the end user against defects in material and workmanship whenpurchased by rental agencies for rental purposes. Within the warranty period, YSIwill repair or replace, at its sole discretion, free of charge, any product that YSIdetermines to be covered by this warranty.To exercise this warranty, call your local YSI representative, or contact YSI CustomerService in Yellow Springs, Ohio at 1 937 767-7241, 800-897-4151 or visit (Support tab) for a Product Return Form. Send the product and proofof purchase, transportation prepaid, to the Authorized Service Center selected byYSI. Repair or replacement will be made and the product returned, transportationprepaid. Repaired or replaced products are warranted for the balance of the originalwarranty period, or at least 90 days from date of repair or replacement.LIMITATION OF WARRANTYThis Warranty does not apply to any YSI product damage or failure caused by:1. failure to install, operate or use the product in accordance with YSI’s writteninstructions;2. abuse or misuse of the product;3. failure to maintain the product in accordance with YSI’s written instructions orstandard industry procedure;4. any improper repairs to the product;5. use by you of defective or improper components or parts in servicing orrepairing the product;6. modification of the product in any way not expressly authorized by YSI.THIS WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES, EXPRESSED ORIMPLIED, INCLUDING ANY WARRANTY OF MERCHANTABILITY OR FITNESSFOR A PARTICULAR PURPOSE. YSI’s LIABILITY UNDER THIS WARRANTYIS LIMITED TO REPAIR OR REPLACEMENT OF THE PRODUCT, AND THISSHALL BE YOUR SOLE AND EXCLUSIVE REMEDY FOR ANY DEFECTIVEPRODUCT COVERED BY THIS WARRANTY. IN NO EVENT SHALL YSI BELIABLE FOR ANY SPECIAL, INDIRECT, INCIDENTAL OR CONSEQUENTIALDAMAGES RESULTING FROM ANY DEFECTIVE PRODUCT COVERED BYTHIS WARRANTY.i

* The warranty period for the non-field rugged cables (605107, 605177, 605108,605178, 605109, 605179) is listed as 6 months. However, the true “working life” ofthese sensors may be 3-9 months depending on storage and usage in solutions otherthan clean aqueous samples.** The warranty for the ammonium, nitrate, and chloride sensors (605104, 605105,605106) is listed as 6 months. However, the true “working life” of these sensors maybe 3-9 months depending on storage and usage in solutions other than clean aqueoussamples.I n t r o duc t i o nThank you for purchasing the YSI Professional Plus (Pro Plus). The Pro Plusfeatures a waterproof (IP-67) case, backlit display and keypad, user-selectablecable options, USB connectivity, large memory with extensive site list capabilities,and a rugged, rubber over-molded case.Reading the entire manual before use is recommended for an overallunderstanding of the instrument’s features.G e t t ing S t ar t edInitial InspectionCarefully unpack the instrument and accessories and inspect for damage.Compare received parts with items on the packing list. If any parts or materialsare damaged, contact YSI Customer Service at 800-897-4151 ( 1 937 767-7241)or the authorized YSI distributor from whom the instrument was purchased.Battery InstallationThe Pro Plus requires (2) alkaline C-cell batteries which are included with thepurchase of a new instrument. Battery life depends on parameters and usage.Under normal conditions, battery life is approximately 80 hours for continuoususe at room temperature. To install or replace the batteries: the instrument over to view the battery cover on the back.Unscrew the four captive battery cover screws.Remove the battery cover and install the new batteries, ensuring correctpolarity alignment on the instrument or the removed cover. (Figure 1)Replace the battery cover on the back of the instrument and tighten the fourscrews. Do NOT over-tighten.Figure 1. Pro Plus with battery cover removed. Noticebattery symbols indicating polarities.ii1

iBatteries must be installed in the instrument even if poweringthe unit via the USB connection. This will retain the correctdate and time if the PC is turned off. If the USB power isdisconnected and there are no batteries in the instrument, thedate and time will need to be reset upon subsequent power on.NOTE - On subsequent battery changes you will have approximately 2 minutesto change the batteries before the clock resets. If the clock resets, the instrumentwill automatically bring up the Date/Time menu the next time it is powered onin order to update this information. This is important, especially if you intendto log data!SetupThe Pro Plus instrument has several compatible field-rugged cable/sensoroptions, each with temperature:Cable:Cable number 60520-xCable number 60530-xCable number 60510-xCable number 6051010-xCable number 6051020-xCable number 6051030-xCable number 6052030-xCable number 605790-xAvailable Sensors:DO/temp (605780 for lab /conductivity/ISE*/ISE*/temp (Quatro**)recommended for lab use or controlled conditions where a more rugged, fieldcable is not necessary. These cables include:Cable number 605107Cable number 605177Cable number 605108Cable number 605178Cable number 605109Cable number 6051791-meter cable; single-junction pH sensor4-meter cable; single-junction pH sensor1-meter cable; single-junction ORP sensor4-meter cable; single-junction ORP sensor1-meter cable; single-junction pH/ORP sensors4-meter cable; single-junction pH/ORP sensorsStandard Pro Series SENSOR InstallationThroughout the manual, the term “sensor” refers to the removable portion orelectrode sensing portion of the cable assembly. For example, the DO sensor orpH sensor is the part that can be removed from a field cable and replaced with anew sensor. The conductivity sensor is not removable from a non-Quatro cablebut still refers to the “sensing” portion and will be referred to as a sensor. Thissection covers most of the sensor installations on a Professional Series cablebulkhead including the following sensors:2003 - PolarographicDO (black)1001 - pH1003 - pH/ORP1005 - Chloride2002 - GalvanicDO (gray)1002 - ORP1004 - Ammonium 1006 - NitrateSee the next section of this manual for installation instructions for the Quatrocable’s Conductivity/Temperature sensor.*ISE (Ion Selective Electrode) notates a port that can accept pH, ORP, Ammonium,Nitrate, Chloride, and, in some cases, a pH/ORP combination sensor.**Cable 605790 will be referred to as a Quatro cable throughout this manual.All cables come in standard lengths of 1, 4, 10, 20, and 30-meters (3.28, 13,32.8, 65.6, and 98.4-feet) with options for special order lengths up to 100-meters(328-feet) on the 60520-x cables. Contact YSI or your local representative foradditional information.Dual sensor bulkhead ports are numbered 1and 2, see figure to the left. Please refer to thefollowing tables to determine correct sensorinstallation into each port of a two port cable.In addition there are several cable options with built in sensors for themeasurement of pH and ORP that are not considered field-rugged (nonreplaceable sensors, less rugged single-junction sensors). These cables are23

1010 dual cablePort 1 OptionsPort 2 OptionspHpHPort 1OptionsPort 2OptionsDO PortOptionsORPORPpHpHpH or pH/ORP*pH or ic 5560DOConductivity/Temperaturesensor onlynitratenitrateORPORPGalvanic DOpH or pH/ORP*pH or pH/ORP*none itratenone (port plug)QuatroCable (pn:605790)* If using a 6051010 cable, a sensor must be installed in port 1 for correctoperation. If you install a pH/ORP combo sensor into a 6051010 cable, ORPwill not be measured. It is not recommended to use a pH/ORP combo sensoron a 6051010 cable.1020 dual cablePort 1 OptionsPort 2 OptionspHPolarographic DOORPGalvanic DOpH or pH/ORPnone (port plug)ammoniumnone (portplug)* If using a Quatro cable, a sensor must be installed in port 1 for correctoperation of port 2. If you install a pH/ORP combo sensor into a Quatrocable, ORP will not be measured. It is not recommended to use a pH/ORPcombo sensor on a Quatro cable.chloridenitratenone (port plug)If using a 1020 cable, install a pH, ORP, pH/ORP, Ammonium, Nitrate orChloride sensor in port 1 and a DO sensor in port 2.iIf using a 605103 pH/ORP combination probe on a6051020 or 6051030 cable you can report both pHand ORP. However, it is recommended to set ISE1 aspH and ISE2 as ORP in the Sensor Setup menu.The Quatro cable bulkheads are labeled1, 2, DO, and CT, see figure to the left. Allsensors except the Conductivity/Temperaturesensor can be installed following the StandardPro Series Sensor Installation instructions.Conductivity/Temperature sensor installationis described in the next section. For ease ofinstallation, YSI recommends that you installa sensor into port 1 first; followed by DOinstallation, then port 2, and lastly C/T.4CT Port OptionsiBefore installing either dissolved oxygen sensor, the instrumentmust be configured for the sensor being installed. Seethe Setup - Dissolved Oxygen section of this manual forinstrument configuration instructions. Failure to do thismay result in damage not covered under warranty.First, ensure both the sensor connector and sensor port on the cable areclean and dry. To connect the sensor, grasp the sensor with one hand and thesensor connection end of the cable (bulkhead) in the other. Push the sensorinto the connector on the cable until it is properly seated and only one o-ringis visible. Failure to properly seat the probe may result in damage. Twist thesensor clockwise to engage threads and finger tighten (Figure 2). Do not use atool. This connection is waterproof. Please refer to the sensor installation sheetthat is included with each sensor for detailed instructions.5

Figure 2. The image on the left shows a clean, dry sensor beingaligned with the bulkhead. On the right, the sensor has beenpushed into the bulkhead and is being screwed into place.Figure 3. The sensor(s) will install directlyin the cable bulkhead. Once installed, thesensor guard will protect the sensor duringsampling (DO cap membrane not shown).Sensor GuardCable BulkheadConductivity/Temperature sensor Installation in aQuatro CableAs mentioned, the installation of the Conductivity/Temperature sensor (model5560) in a Quatro cable is different from all other Pro Series sensor installations.Follow these instructions when installing a conductivity/temperature sensor ina Quatro cable:2.Locate the C/T port and, if replacing, remove the old sensor using theinstallation tool to loosen the stainless steel retaining nut. Once the stainlesssteel retaining nut has been completely unscrewed from the bulkhead,remove the old sensor from the bulkhead by pulling the sensor straight outof the bulkhead.Apply a thin coat of o-ring lubricant (supplied with the sensor) to the o-ringson the connector side of the new sensor.iiDo not cross thread the sensor nut. Seat nut onface of bulkhead. Do not over tighten.Please refer to the sensor installation sheet that is included with the conductivity/temperature sensor for detailed instructions.Figure 4. Installation tool used totighten stainless steel retaining nut of5560 conductivity/temperature sensor.Sensor1.3. Align the connectors of the new sensor and the port. With connectorsaligned, push the sensor in towards the bulkhead until you feel the sensorseat in its port. You will experience some resistance as you push the sensorinward, this is normal4. Once you feel the sensor seat into the port, gently rotate the stainless steelsensor nut clockwise with your fingers, Do not use the tool.5. The nut must be screwed in by hand. If the nut is difficult to turn, STOP, asthis may indicate cross threading. If you feel resistance or cross threadingat any point, unscrew the nut and try again until you are able to screw thenut down completely without feeling any resistance. Damage to your cable/sensor may occur if you force the parts together.6. Once completely installed, the nut will seat flat against the bulkhead. Atthis point, use the tool that was included with the sensor to turn the nut anadditional ¼ to ½ turn so it cannot come loose (figure 4). DO NOT overtighten.Installing Port Plugs in Unused PortsAs necessary, install a port plug into any port that does not have an installedsensor. This will protect the bulkhead from water damage. Port plugs and atube of o-ring lubricant are included with all Quatro cables. These items can beordered separately if needed. To install a port plug, apply a thin coat of o-ringlubricant to the two o-rings on the port plug. After application, there shouldbe a thin coat of o-ring lubricant on the o-rings. Remove any excess o-ringlubricant from the o-ring and/or port plug with a lens cleaning tissue. Next,insert the plug into an empty port on the bulkhead and press firmly until seated.Then, turn the plug clockwise to engage the threads and finger-tighten until theplug is installed completely. Do not use a tool to tighten the plug.Visually inspect the port for moisture. If moisture is found,it must be completely dried prior to sensor installation.67

Connecting the Cable to an InstrumentKeypad2To connect a cable, align the keys on the cable connector to the slots on theinstrument connector. Push together firmly, then twist the outer ring until itlocks into place (figure 5). This connection is water-proof.3141351261110789Figure 5. Note the keyed connector. The cable and instrumentconnectors can only be mated once the keys are properly aligned.NumberiWhen a sensor is not installed, the sensor and cable sensorconnectors are NOT water-proof. Do not submerge the cablewithout a sensor or port plug installed in all available ports.When the cable is disconnected, the cable’s instrument connectorand the connector on the instrument maintain an IP-67 rating.Sensor StorageThe cable assembly is supplied with a storage container, or sleeve, that installson to the cable. The container is used for short-term storage (less than 30 days).Be sure to keep a small amount of moisture (tap water) in the container duringstorage. This is done to maintain a 100% saturated air environment which isideal for short-term sensor storage (see Care, Maintenance, and Storage for moredetailed information). Do not submerge the sensors in an aqueous solution.The intent is to create a humid air storage environment.8KeyDescription1SystemOpens System Menu from any screen.Use to adjust system settings.2SensorOpens Sensor Menu from any screen.Use to enable sensors and display units.3CalibrateOpens Calibrate Menu from any screen.Use to calibrate all parameters except temperature.4FileOpens File Menu from any screen.Use to view data and GLP files, set upsite and folder lists, and delete data.5BacklightPress to turn the instrument backlight on andoff and to adjust the display contrast whenpressed with the left or right arrow key.9

NumberKeyMain DisplayDescription6Right ArrowUse to navigate right in alpha/numeric entryscreens. Can be pressed simultaneously withBacklight key to increase display contrast.7Down ArrowUse to navigate through menus and to navigatedown in alpha/numeric entry screens.8PowerPress to turn the instrument on.Press and hold for 3 seconds to turn off.9HelpPress to receive hints & tips during operation.10EnterPress to confirm selections, includingalpha/numeric key selections.11Left ArrowUse to navigate left in alpha/numeric entry screens.Press to return to previous menu in allscreens except alpha/numeric entry.Can be pressed simultaneously with Backlightkey to decrease display contrast.12Exit/EscapeExits back to Run Screen.When in alpha/numeric entry screen,escapes to previous menu.13Up ArrowUse to navigate through menus and to navigateup in alpha/numeric entry screens.Press the Power keyto turn the instrument on. The instrument will brieflydisplay the splash screen with the YSI logo then go directly to the main runscreen. The first time the instrument is powered up or if the instrumenthas had a battery change (with batteries removed for more than 2 minutes),you will need to set the date and time. Follow the instructions underSystem Menu Date/Time.The display at the left shows the run mode (maindisplay) with temperature in C, barometerin mmHg, DO in % and mg/L, and pH as thereported parameters. The date, time and batterylevel are indicated at the bottom of the screen.The logging preference of Log One Sample at atime is indicated at the top of the screen.This screen also shows the message line towardsthe bottom of the display above the date andtime. In this case, it doesn’t show a messagebut messages will appear frequently to indicatecalibration steps, set date and time, etc.will show up on theA USB symbolbottom of the display when connected to a PCthrough USB with the communications saddle. The instrument will display fullbattery power when it is receiving power through the USB connection.iContrast – the contrast adjustment can be accomplished by pressingthe backlight key and the left or right arrow key at the same time.Menu LayoutPress Escat anytime in the menus to escape back to the Run screen. Thecan be used to go back to the previous menu in all screens exceptleft arrowalpha/numeric entry screens. You must use Esc to get out of the alpha/numericscreens if you want to exit before finishing or without saving changes. Functionsthat are enabled appear as a circle with a dot or a box with a check mark Q.Disabled functions appear as a circle only or an empty P.1011

S ys t em M enuAlpha/Numeric EntryPress Systemmenu access any of the followingThe System menu will allow you to access thesetup options of the instrument including; Date/Time, GLP, Language, Radix Point, Logging,Auto-Shutoff, Backlight, SW (Software)Version, Serial #, and Unit ID. Any item with[brackets] shows the current setting inside thebrackets. For instance, in the example at theleft, Radix Point is currently set to [Decimal].The brackets will also give a quick visual clue asto what items can be changed.The numeric screens will display numbers only (shown on the left). Alpha/numeric screens will display numbers across the top and letters along thebottom rows (shown on the right). Letters appear as a common keyboardarrangement.Date/TimeHighlight Date/Time from the System menu.Press enter to select.When an alpha or numeric character is required, the screen will show the alpha/numeric entry screen. To select a character, highlight it by using the arrow keysto move the highlight box over the desired selection. Then, press Enter on thekeypad to confirm the selection. After confirming the selection, it will appearin the line at the top of the display.Date Format – Highlight and press enter toopen a sub menu for selecting the preferreddate format: YY/MM/DD, MM/DD/YY,DD/MM/YY, or YY/DD/MM.For capital letters or lower case entry, highlight “SHIFT” and press Enter on thekeypad to change the characters from upper to lower case.Date – Highlight and press enter to use thenumeric entry screen to set the correct date.Time Format – Highlight and press enter toopen a submenu to select the preferred timeformat from 12-hour or 24-hour.To delete the entire line of the current entry, highlight ) and press Enter on thekeypad. The ! symbol functions as a backspace key in the alpha/numeric entryscreens by deleting one character at a time. Use the “SPACE” function to add aspace between characters.Time – Highlight and press enter to use thenumeric entry screen to set the correct time.When you have finished entering the correct information (16 character max),highlight ENTER at the bottom of the screen and press Enteronthe keypad to confirm.ikey cannot be used to escape to the previous menu fromThekey to goan alpha/numeric entry screen. Instead, use theback to the previous menu when in alpha/numeric entry screens.12iThe date and time will need to be reset if a batterychange takes longer than 2 minutes. When this occurs,the Date/Time menu will automatically appear uponpower up and require you to set the date and time.13

GLPThe GLP or ‘Good Laboratory Practice’ file saves detailed informationabout calibrations. It also includes diagnostic information about the sensors.Calibrations are logged into a file, the GLP, for later review as needed. A singleGLP file is utilized to store all calibration records and is capable of storing 500records. Once the GLP file is full, the instrument will begin to overwrite theoldest record with each new calibration record.iIn order to keep all of your GLP records, periodically downloadthe GLP to Data Manager and export it to another program.Otherwise, the unit will overwrite the oldest record oncethe memory is full. Also, since Data Manager saves GLPfiles under the Unit ID, you must periodically export andrename the GLP file on your PC or it will be overwritteneach time you upload the GLP file from the instrument.Several calibration parameters are saved for each calibration record includingoptional ones that can be enabled by the user. Standard parameters includedate/time stamp, calibration method, and sensor information. Optional, userselectable parameters include User ID, Probe ID, and User Fields 1 and 2.The sensor specific information that is saved with each calibration point isdifferent for each sensor. The sensor specific values saved are:ConductivityMethod (Spec Cond, Cond, Salinity)Cal Value (value of calibration solution)Sensor Value (Cell Constant)Temperature Reference (User selected in Sensor Setup menu)Temperature Compensation Coefficient %/ C (User selected in Sensor Setupmenu)TDS Constant (User selected in Sensor Setup menu)TemperatureCal Cell ConstantCalibrate StatusDOMethod (%, mg/L)Cal ValueSensor Value (Sensor Current)Sensor Type (Polarographic/Galvanic)Membrane Type (Teflon Black, PE Yellow, PE Blue)Salinity Mode (user entered value if in Manual Salinity Mode)TemperatureBarometerCalibrate StatuspH (up to 6 calibration points)Buffer ValueSensor Value (mV)TemperatureSlope (mV/pH)Slope (% of ideal)Calibrate StatusORPCal Solution ValueSensor ValueTemperatureCalibrate StatusAmmoniumBuffer ValueSensor Value (mV)TemperatureCalibrate StatusChlorideBuffer ValueSensor Value (mV)TemperatureCalibrate StatusNitrateBuffer ValueSensor Value (mV)TemperatureCalibrate Status1415

An example of a GLP record(Operation performed is

Service in Yellow Springs, ohio at 1 937 767-7241, 800-897-4151 or visit www. (Support tab) for a Product Return Form. Send the product and proof of purchase, transportation prepaid, to the Authorized Service Center selected by YSI. Repair or replacement will