Instructions for UseCyAn ADPHigh-speed Analyzer0000050KNovember 2014Beckman Coulter, Inc.250 S. Kraemer Blvd.Brea, CA 92821 U.S.A.

CyAn ADPInstructions For UseDocument 0000050 (November 2014)Find us on the World Wide Web at:www.beckmancoulter.comThis manual is not a substitute for the detailed operatortraining provided by Beckman Coulter or for otheradvanced instruction. Contact a Beckman CoulterRepresentative in the event of instrument malfunction.

Revision HistoryInitial Issue2004Software Version Summit 4.1Revision G, 06/2008Software Version Summit 4.3Change labeling to Beckman Coulter name and logo. Update consumables list, Laser Delayprocedure, Summit Overview, Six-color compensation experiment, and document organization.Revision H, 01/2009Software Version Summit 4.3Change reference to Worklist Builder Quick Start Guide.Revision J, 05/2009Software Version Summit 4.3Note: We do not use the letters I or O for revisions because they can be confused with numbers.Change reference to Windows XP.Revision K, 11/2014Software Version Summit 4.4Updates were made to the company corporate address.0000050K-iii

Safety NoticeRead all product manuals and consult with Beckman Coulter-trained personnel before attemptingto operate instrument. Do not attempt to perform any procedure before carefully reading allinstructions. Always follow product labeling and manufacturer’s recommendations. If in doubt asto how to proceed in any situation, contact your Beckman Coulter representative.Beckman Coulter, Inc. urges its customers to comply with all national health and safety standardssuch as the use of barrier protection. This may include, but is not limited to, protective eyewear,gloves, and suitable laboratory attire when operating or maintaining this or any other matedlaboratory analyzer.Alerts for Warning and CautionWARNINGWARNING indicates a potentially hazardous situation which, if not avoided, couldresult in death or serious injury. May be used to indicate the possibility oferroneous data that could result in an incorrect diagnosis (does not apply to allproducts).CAUTIONCAUTION indicates a potentially hazardous situation, which, if not avoided, mayresult in minor or moderate injury. It may also be used to alert against unsafepractices. May be used to indicate the possibility of erroneous data that couldresult in an incorrect diagnosis (does not apply to all products).0000050K-v

Safety NoticeAlerts for Warning and CautionWARNINGRisk of operator injury if: All doors covers and panels are not closed and secured in place prior to andduring instrument operation. The integrity of safety interlocks and sensors is compromised. Instrument alarms and error messages are not acknowledged and acted upon. You contact moving parts. You mishandle broken parts. Doors, covers and panels are not opened, closed, removed and/or replacedwith care. Improper tools are used for troubleshooting.To avoid injury: Keep doors, covers and panels closed and secured in place while theinstrument is in use. Take full advantage of the safety features of the instrument. Acknowledge and act upon instrument alarms and error messages. Keep away from moving parts. Report any broken parts to your Beckman Coulter Representative. Open/remove and close/replace doors, covers and panels with care. Use the proper tools when troubleshooting.CAUTIONSystem integrity could be compromised and operational failures could occur if: This equipment is used in a manner other than specified. Operate theinstrument as instructed in the product manuals. You introduce software that is not authorized by Beckman Coulter into yourcomputer. Only operate your system’s software with software authorized byBeckman Coulter. You install software that is not an original copyrighted version. Only usesoftware that is an original copyrighted version to prevent viruscontamination.CAUTIONIf you purchased this product from anyone other than Beckman Coulter or anauthorized Beckman Coulter distributor, and, it is not presently under a BeckmanCoulter service maintenance agreement, Beckman Coulter cannot guarantee thatthe product is fitted with the most current mandatory engineering revisions orthat you will receive the most current information bulletins concerning theproduct. If you purchased this product from a third party and would like further-vi0000050K

Safety NoticeInstrument Safety Precautionsinformation concerning this topic, call your Beckman Coulter Representative.Instrument Safety PrecautionsThe CyAn high-speed cell analyzer has been engineered with safety as one of its primary features.Safety of the operator, field service personnel, bystanders, and of valuable samples, is paramount toBeckman Coulter’s commitment to high performance design and engineering.This section explains some general safety and hazard symbols and necessary precautions operatorsof the CyAn should follow during operation. Engineering controls have been put in place to protectthe operator, and deliberate misuse of the instrument or its instructions may result in unintentionalharm. Please follow all safety and hazard instructions as directed in this manual.SymbolsBelow are the symbols used and their corresponding meanings, which can be found on theinstrument and throughout this manual.Safety SymbolsElectrical Shock — Risk of Electric ShockLaser Irradiation — Avoid looking directly into laser, as it may cause permanent eye damage.Biohazard — Biological Hazard/RiskCaution — Important; Attention; Refer to Accompanying DocumentationElectrical SafetyThe Beckman Coulter instrument product line conforms to international regulations encompassingthe accessibility of high voltages by the user. In the United States, each flow cytometer ismanufactured to comply with applicable regulations from the American National StandardsInstitute (ANSI), the Occupational Safety and Health Administration (OSHA), and various stateregulatory organizations. Internationally, each CyAn ADP High-Performance Flow Cytometer ismanufactured to comply with the International Electrotechnical Commission's Standard for SafetyRequirements for Electrical Equipment for Measurement, Control and Laboratory Use (IEC 61010-1)and subsequent amendments.0000050K-vii

Safety NoticeInstrument Safety PrecautionsOptical/Laser SafetyLaser Product Hazard ClassificationThe intent of laser hazard classification is to provide clear distinction of the laser, or laser productproperties, and the hazards to users so appropriate protective measures can be taken. CyAn ADP isa Class 1 laser product per 21 CFR 1040 and EN60825; meaning operators are not exposed to harmfullevels of laser irradiation during normal operation. During times of service and/or repair, lasersafety control measures for Class 3B and/or 4 lasers shall be followed.NOTE Because the CyAn ADP has been designed to operate as a Class 1 Laser Device, the instrument mustbe operated with all light containment tubes in place and all protective light seals intact. Most lasercomponents of the CyAn ADP flow cytometer are Class IIIB to Class IV. As such, these lasers have thepotential to cause injury. There is one small viewing window inside the CyAn ADP, but its purpose is to beused only by trained Beckman Coulter personnel during service or repair. A safety interlock preventsoperators from viewing laser light through this viewing window under normal operating conditions.Certification of regulatory compliance of these lasers often requires significant involvement from theinstitutional or company laser-safety officer. Beckman Coulter does not provide regulatory certification forthe individual lab, but can offer assistance in laser safety matters.All access plates and other user accessible points of potential laser exposure are clearly designated onthe CyAn ADP instrument. Interlocks are designed to prevent accidental irradiation of the operator. Theuser should not defeat these interlocks.Any questions or concerns regarding laser safety of the CyAn ADP should be directed to Beckman CoulterCustomer Care.Laser Safety Label-viii0000050K

Safety NoticeInstrument Safety PrecautionsCyAn ADP Interior Laser Safety Label LocationsBiohazard SafetyIMPORTANT If any hazardous organism, material, or agent is used in the instrument, the site operator orPrincipal Investigator is responsible for informing Beckman Coulter in writing of those hazards beforereceiving service or repair. This includes a list of all pathogenic cell lines, hazardous reagents, radioactivematerial, or agents with a BSL Level II or higher. This information will be kept confidential and will be usedto inform Beckman Coulter Field Service Representatives of any hazards prior to visiting any CyAn site.Failure to report this information may delay service on an instrument. Safety of the user as well as safetyof Beckman Coulter employees is of overriding importance. Proper decontamination procedures must befollowed for all applicable returned parts.Universal precautions should be followed when handling samples and waste fluid containingsamples: Gloves, a laboratory coat, and eye protection should be worn whenever handling samples. Waste fluid may contain hazardous levels of biological contamination. Gloves, a laboratory coatand eye protection should be worn whenever exposure to waste fluid is possible. To ensure inactivation of biological organisms in the waste tank, an appropriate type andquantity of an EPA registered disinfectant should be placed in the tank initially upon use, andevery time the waste tank is emptied and reinstalled.For additional information on laboratory biosafety, review the U.S. Department of Health andHuman Services, Centers for Disease Control document, Biosafety in Microbiological and BiomedicalLaboratories.Waste TankThe reusable waste tank is located on the right side of the SMS cart and is labeled with a large blackand orange biohazard symbol on the front. Emptying waste fluid generated from the CyAn ADPcould be a daily occurrence for some operators. It is expected that all operators have undergoneproper blood-borne pathogens training provided by the institution of use, or similar authority,prior to using the CyAn. It is pertinent that the appropriate type and quantity of disinfectant is0000050K-ix

Safety NoticeInstrument Safety Precautionsplaced in the waste tank to ensure effective inactivation of the biologics in use when the tank is full.Please see APPENDIX A, for a list of approved cleaners and disinfectants for use with the CyAn ADP.Please use caution when emptying the waste tank to avoid splashing of the waste fluid. PersonalProtective Equipment (PPE) should be worn at all times when using any part of the CyAn ADP,including emptying the waste tank. Please contact Beckman Coulter Customer Care if there are anyquestions or concerns about handling waste fluid generated by the CyAn ADP.Sample WasteExposure to sample waste fluid from clogs in the waste line may occur; however, this is rare. If a clogoccurs, exposure to biohazards is likely. PPE should be worn at all times when using the CyAn ADPto protect the operator from unexpected hazards. If exposure to biohazardous material occurs dueto a fault of the CyAn ADP, Beckman Coulter Customer Care should be contacted immediately.SamplesSample handling is a normal part of the use of the CyAn ADP. It is expected that all operators haveundergone proper blood borne pathogens training provided by their institution, or similarauthority, prior to using the CyAn ADP. PPE should be worn at all times when using any part of theCyAn ADP. Samples of any kind should not be handled without wearing gloves. Caution should beused when handling any biohazardous samples. Any reagents used on the instrument, or in thepreparation of samples for use on the CyAn ADP, should be handled by professional users only.Sample O-ringThere is an o-ring located in the top interior of the sample injection area. The purpose of this o-ringis to create a seal when a test tube is placed on the sample probe, which results in pressurization ofthe tube. Over time this o-ring may lose its elasticity or become damaged from repeated contactwith polypropylene test tubes. Deterioration of this o-ring may cause test tubes to be loose whenplaced on the sample probe, leading to the potential for test tubes filled with biohazardous samplesfalling off, causing exposure to the operator or other laboratory personnel. This occurrence is notlikely, but if an operator experiences this condition, Beckman Coulter Customer Care should becontacted immediately so the o-ring can be replaced.Sample Waste ManifoldWaste fluid is continuously generated when running the CyAn ADP and is a normal function of thisinstrument. This waste fluid is routed through a manifold located within the SMS cart. If this partmalfunctions, a backup of fluid may occur, resulting in waste fluid dripping through a small hole inthe top corner of the sample injection area. Because this waste fluid may contain biohazardousmaterial, caution should be used if this fault is identified; however, this occurrence is highlyunlikely. PPE should be worn at all times when using any part of the CyAn ADP to ensure protectionof the operator from all potential hazards. If an operator identifies such a leak, Beckman CoulterCustomer Care should be contacted immediately.AerosolsThe CyAn ADP is a flow cytometry analyzer and has no sorting capabilities. No aerosols aregenerated under normal operating conditions. In the event of extreme over pressurization of thesystem, there is a remote chance that sample and/or waste fluid could exit orifices at a rate whichcould potentially create aerosols; however, this has never been experienced. If at any time anoperator identifies a potential over pressurization problem, Beckman Coulter Customer Care shouldbe contacted immediately.-x0000050K

Safety NoticeInstrument Safety PrecautionsSample ProbeThe sample probe is constructed of a stainless steel tube with one end slightly squared off. Directcontact with the end of the probe could cause an abrasion or cut. Because biohazardous samples arerun through the sample probe, there is a chance that if the skin is broken due to direct contact withthe end of the sample probe, an operator could be directly exposed to biohazardous material. Usecaution when loading and unloading samples on the probe. PPE should be worn at all times whenusing any part of the CyAn ADP. If injury related to the sample probe occurs, Beckman CoulterCustomer Care should be contacted immediately.General SafetyTo protect the health, environment, and safety of CyAn ADP sites and their users, the followinginformation should be reviewed by all operators. The CyAn ADP is intended for Professional Use Only. All operators should be trained on theproper use and limitations of the instrument prior to its operation. Wear appropriately sized gloves providing good manual dexterity to reduce the likelihood ofskin pinches and abrasions. Protect the skin and eyes whenever handling chemicals of any kind, regardless of how benignthey may appear. Summit workstations include a keyboard interface. Evaluate the ergonomic suitability of thelocation of the keyboard and the user to avoid injury. Cords and cables may be located on the floor around the unit, be aware that they can cause atripping hazard. The Sheath Management System (SMS) is attached to the CyAn ADP head unitby an 8 foot (2.4 meters) umbilical cord. It is suggested that the SMS cart be placed underneatha laboratory bench to keep it out of the way of foot traffic. If this is not possible, it isrecommended that the SMS be placed where it will not pose a tripping hazard. It is suggestedthat the umbilical cord be run behind the laboratory bench, if possible, to keep the cord fromcreating a tripping hazard. If the cord must be run down in front of the laboratory bench to theSMS, use caution when operating or walking past the CyAn ADP. It is suggested that hazard tapebe placed on these items to make them highly visible. Always check with the site safety officer for correct disposal of waste products. Use proper lifting techniques, or seek assistance when handling full tanks. To reduce thelikelihood of back injury, empty waste at least once per day. The lid of the CyAn ADP is on hinges that should keep the lid in the up position when open. It isrecommended by Beckman Coulter that the lid not be left open for extended periods of time orbe opened and closed repeatedly. Excessive wear on the hinges over time may cause the lid tofall unexpectedly. Use caution when the lid is open and do not leave the instrument unattended.If the lid of the CyAn ADP becomes loose and will not stay up on its own, contact BeckmanCoulter Customer Care so it can be repaired.Electromagnetic InformationThis equipment has been tested and found to comply with the limits for a Class A digital device,pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable protectionagainst harmful interference when the equipment is operated in its installation. This equipment0000050K-xi

Safety NoticeInstrument Safety Precautionsgenerates, uses, and can radiate radio frequency energy. If not installed and used in accordance withthe instruction manual this equipment may cause harmful interference to radio communications.If this equipment does cause harmful interference the user will be required to correct theinterference. This Class A digital apparatus complies with Canadian ICES-003.-xii0000050K

ContentsRevision History, iiiSafety Notice, vIntroduction, xxiiiCHAPTER 1:Installation, 1-1General Laboratory Information, 1-1CyAn ADP Installation Specifications, 1-1Technical and Instrument Specifications, 1-3CHAPTER 2:System Overview, 2-1Overview of the CyAn ADP Analyzer, 2-1CyAn ADP Features, 2-2Walk-up Operation, 2-2Bench Top Configuration, 2-2Summit Software, 2-2Safety, 2-2CyAn ADP Subsystems, 2-2Fluidics Subsystem, 2-4Sheath Management System (SMS) Indicators, 2-5Optics Subsystem, 2-6Electronics Subsystem, 2-9Peripheral Devices, 2-9Software, 2-9Summit Features, 2-10CHAPTER 3:Summit Software Overview, 3-1Overview, 3-1Summit Software Database, 3-2Summit Software Screen Overview, 3-4Summit Software Control Panel, 3-4Acquisition Tab, 3-5Acquisition Sample Panel, 3-6How to Change Acquisition Settings, 3-7-xiii

ContentsLoading an Existing Protocol, 3-9Creating Protocols, 3-9Switching Protocols, 3-10Acquiring Data in Summit Software, 3-10Saving Acquired Data, 3-10Cycle Mode, 3-11Sample Tab, 3-13Auto Load from Sample, 3-13Auto Compensation Wizard for Single Positive Controls, 3-14Applying VisiComp, 3-17FCS Keywords, 3-19Histogram Tab, 3-21Creating Histograms and Dot Plots, 3-21Maximize Dot Plots and Histograms, 3-22Change Axis Parameters, 3-22Change Axis Labels, 3-23View Statistics in Histograms, 3-23Edit Statistics Displayed in Histograms, 3-24Create Regions in Histograms, 3-25Renaming Regions, 3-25Copy and Paste Regions, 3-25Manually Scaling Data, 3-26Contouring Data, 3-26Exporting Histograms to Word, 3-26Multi File Display, 3-26Create Overlays, 3-27Gate Logic Tab, 3-28Setting a Gate from a Single Region, 3-28Setting a Serial Gate, 3-30Gate Logic Builder, 3-32Color Gating, 3-34Layout Tab, 3-35Workspace Page Setup, 3-35Workspace Page Navigator, 3-36Shortcut Keys, 3-36CHAPTER 4:Startup and Shutdown, 4-1CyAn ADP Startup, 4-1All CyAn ADP Operators, 4-1How to Startup CyAn ADP, 4-1How to Perform a Daily Decontamination Procedure, 4-4Shutdown Procedure, 4-5CHAPTER 5:Instrument Validation, 5-1CyAn Instrument Alignment Example, 5-1How to Run a General Instrument Alignment Protocol, 5-1-xiv

ContentsCHAPTER 6:Cleaning and Maintenance, 6-1CyAn ADP Maintenance, 6-1Daily Preventive Maintenance, 6-1Weekly Preventive Maintenance, 6-1How to Maintain Reusable Sheath Containers, 6-1How to Maintain the Head Unit and SMS Cart, 6-2How to Clean Fluidics Lines, 6-2Monthly Preventive Maintenance, 6-2How to Perform System Decontamination, 6-3How to Perform Laser Interlock Maintenance, 6-4Biannual Maintenance Procedure, 6-4How to Change Sheath and Waste Containers, 6-5How to Replace Cleaner Fluid, 6-6CHAPTER 7:Troubleshooting, 7-1CyAn ADP Instrument Troubleshooting, 7-1Possible Error Conditions in the SMS, 7-1APPENDIX A:Approved Cleaners and Disinfectants, A-1Overview, A-1Cleaners, A-1Disinfectants for Use in Sample Line, A-1Disinfectants for Use in Sheath Line, A-1Disinfectants for Use in the Waste Tank, A-2APPENDIX B:Consumables, B-1Consumables, B-1APPENDIX C:Compensation Background Information, C-1Compensation, C-1Six-color Experiment Compensation, C-2Setting up for Sample Acquisition, C-3Creating Histograms for Six-color Experiment Compensation, C-4Manual Compensation, C-7Setting Spillover Percentage for Compensation, C-8Running the Second Single-color Sample, C-9Running the Remaining Samples, C-11Running the All-stains Sample, C-16Putting Compensation Into Practice, C-17CD3 and T-Lymphocytes, C-18CD20 and B-Lymphocytes, C-18CD4 /CD3 T-Lymphocytes (T-Helper cells), C-19CD8 /CD3 T-Lymphocytes (T-Cytotoxic/Suppressor cells), C-19Natural Killer Lymphocytes, C-20-xv

ContentsAPPENDIX D:Laser Delay, D-1Setting Laser Delay, D-1APPENDIX E:Symbols, E-1Symbol Definitions, E-1Abbreviations, Abbreviations-1References, References-1Trademarks, Trademarks-1Beckman Coulter, Inc.Customer End User License Agreement, Warranty-1Related Documents-xvi

FiguresFigures1.1Power Configuration and Consumption, 1-22.1CyAn ADP Analyzer, 2-12.2CyAn ADP Functional Block Diagram, 2-32.3CyAn ADP Fluidics Block Diagram, 2-42.4SMS Section of the Instrument Control Panel, 2-52.5Optics Block Diagram, 2-62.6CyAn Flow Cell Interrogation Point, 2-72.7Detector Block and Optical Filter Layout, 2-82.8CyAn ADP Filter Stick, 2-82.9Instrument Control Panel, 2-102.10Sample Parameters Panel, 2-103.1Select a New Database, 3-13.2Create a New Database, 3-23.3Browse to an Existing Database, 3-23.4Select an Existing Database, 3-33.5Summit Software Screen Overview, 3-43.6Summit Software Control Panel (see number 3. onFigure 3.5), 3-43.7Acquisition Tab, 3-53.8Acquisition Sample Panel, 3-63.9Acquisition Sample - Accessing Edit View, 3-63.10Acquisition Sample - Editing Example, 3-63.11Set Event Threshold Percentage, 3-73.12Set Event Trigger Parameter, 3-73.13Change Parameter Name, 3-83.14Set Voltage for a Parameter, 3-93.15Set Gain for the Voltage, 3-93.16Start Acquiring Data, 3-103.17Setting the Cycle Amount for Cycle Mode, 3-113.18Enabling Cycle Mode, 3-123.19Sample Tab, 3-133.20Auto Load Compensation Matrix from FCS File, 3-133.21Select Auto Compensate, 3-153.22Auto Comp Sample Dialog, 3-153.23Auto Compensate Wizard, 3-16-xvii

Figures-xviii3.24Single Control Sample Dot Plots, 3-163.25Apply VisiComp, 3-173.26VisiComp Example, 3-183.27Adjust VisiComp, 3-183.28Add/Remove Keywords 1, 3-193.29Add/Remove Keywords 2, 3-203.30Histogram Tab, 3-213.31Creating Histograms and Dot Plots, 3-223.32Change Axis Parameters, 3-233.33Show Statistics in Histograms, 3-233.34Edit Statistics Displayed in Histograms, 3-243.35Edit Statistics Displayed in Histograms, 3-243.36Create a Region in a Bivariate Histogram, 3-253.37Manually Scaling Data, 3-263.38Gate Logic Tab Displaying an Alignment Protocol and Gating Scheme, 3-283.39Set Gate 1, 3-293.40Set Gate 2, 3-293.41Set Serial Gate 1, 3-303.42Set Serial Gate 2, 3-313.43Set Serial Gate 3, 3-313.44Set Serial Gate 4, 3-323.45Set Serial Gate 5, 3-323.46Gate Logic Builder, 3-333.47Edit Gate Expression 1, 3-333.48Edit Gate Expression 2, 3-343.49Gate Logic Builder and Gate Scheme Panels, 3-343.50Layout Tab Displaying an Alignment Protocol, 3-353.51Workspace Setup Panel, 3-353.52Workspace Page Navigator, 3-364.1Open a Summit Database, 4-14.2CyAn Instrument Control Panel, 4-24.3CyAn ADP Instrument with Sample Tube Loaded and LeverOpen, 4-34.4Summit Preferences, 4-55.1Example Alignment Protocol, 5-25.2CyAn Instrument Control Panel, 5-37.1Low Sheath Fluid Warning, 7-2

Figures7.2Empty Sheath Fluid Message, 7-27.3Quick Connect Error Message, 7-27.4SMS Fluid Levels are Suitable for Operation, 7-37.5SMS Low Sheath and Cleaner Levels, 7-37.6SMS High Waste Level, 7-37.7SMS Sheath and Cleaner Empty, 7-47.8SMS Waste Full, 7-4C.1FL1, FL2 Spectra Diagram, C-1C.2Unstained Sample Tube #1, C-3C.3Region R1 Around Lymphocytes, C-4C.4Six-color Compensation Unstained Sample (Ungated), C-5C.5Six-color Compensation Unstained Sample (GateApplied), C-5C.6Unstained Sample to Verify PMT Settings, C-6C.7Single-stain Sample FITC Uncompensated, C-7C.8Single-stain Sample FITC Compensated, C-8C.9Single-stain Sample PE Uncompensated, C-9C.10Single-stain Sample PE Compensated, C-10C.11Spectral Emission Curves for FITC and PE, C-10C.12Single-stain Sample PE-Cy5 Uncompensated, C-11C.13Single-stain Sample PE-Cy5 Compensated, C-12C.14Single-stain Sample APC Uncompensated, C-12C.15Single-stain Sample APC Compensated, C-13C.16Single-stain Sample Cascade Yellow Uncompensated, C-13C.17Single-stain Sample Cascade Yellow Compensated, C-14C.18Single-stain Sample Pacific Blue Uncompensated, C-15C.19Single-stain Sample Pacific Blue Compensated, C-15C.20All-stains Sample, C-16C.21Compensated CD3 Pacific Blue vs. Compensated CD20 PECy5 Gated Through R1, C-18C.22Compensated CD3 Pacific Blue vs. Compensated CD4FITC, C-19C.23Compensated CD3 Pacific Blue vs. Compensated CD8APC, C-19C.24Compensated CD3 Pacific Blue vs. Compensated CD56PE, C-20D.1Laser Delay Adjustment Window, D-2D.2Secondary Laser Signal Prior to Laser DelayAdjustment, D-2-xix

Figures-xxD.3Maximized Signal for Secondary Laser, D-3D.4Tertiary Laser Signal Prior to Laser Delay Adjustment, D-3D.5Maximized Signal for Tertiary Laser, D-3

TablesTables1.1CyAn ADP General Requirements, 1-11.2Auxiliary Components, 1-21.3Operating Environment, 1-21.4Instrument Performance, 1-31.5Excitation Optics, 1-31.6Detectors and Filters (User Selected), 1-31.7Signal Processing, 1-31.8Fluidics, 1-31.9Summit Workstation, 1-41.10CyAn ADP Enclosure, 1-41.11Auxiliary Components, 1-41.12Safety, 1-51.13Operating Environment, 1-51.14System Utility Requirements and Fusing, 1-53.1Shortcut Keys and Functions, 3-364.1Target CVs for CyAn ADP, 4-47.1CyAn ADP Troubleshooting Matrix, 7-17.2CyAn ADP Instrument Control Panel Indicators inSummit, 7-4B.1Beads, B-1B.2Sheath, B-1B.3Maintenance, B-1C.1Six-color Experiment Compensation, C-2E.1Symbol Definitions, E-1-xxi

IntroductionHow to Use Your ManualScopeThe CyAn ADP Instructions for Use manual contains basic information regarding the use andoperation of the CyAn ADP and assumes you have received basic training on the instrument. Pleasecontact your Beckman Coulter Representative for information not provided in this manual. Thismanual does not provide instructions for the installation or upgrade of hardware because suchactions must be provided by a Beckman Coulter Representative.Intended UseThis instrument is intended for research use only.Use the Instructions For Use manual for the day-to-day running of your instrument andworkstation. Go through the detailed step-by-step procedures of startup, quality control (QC),running samples, analyzing data, printing reports, reviewing QC data, and shutdown beforeoperating the instrument. This manual contains, safety and troubleshooting information, as well asprocedures for cleaning the instrument and replacing some components.About this ManualThe information in your Instructions for Use manual is organized as follows:Chapter 1, InstallationProvides system specifications, lab environment requirements, and the instrument installationrecommendation.Chapter 2, System OverviewProvides an overview of CyAn ADP features, system architecture, and subsystems.Chapter 3, Summit Software OverviewProvides basic information regarding the features in Summit software.0000050K-xxiii

IntroductionConventionsChapter 4, Startup, Alignment and ShutdownProvides information on starting, and shutting down the instrument.Chapter 5, Performance Validation and CalibrationContains an example validation and calibration procedure.Chapter 6, Cleaning and MaintenanceDescribes the cleaning procedures for daily cleaning as well as the biannual and yearlydecontamination process.Chapter 7, Troubleshooting ProceduresProvides a basic troubleshooting matrix and a list of possible error messages.Appendix A, Approved Cleaners and DisinfectantsContains a list of cleaners and disinfectants that can be used on the CyAn analyzer.Appendix B, ConsumablesContains a list of consumables to be used with the CyAn analyzer.Appendix C, Compensation Background InformationProvides information on how to resolve actual intensities from each antibody conjugate in amulticolored sample. Includes an example of compensation for a six-color experiment.Appendix D, Laser DelayProvides instructions on how to set Laser Delay.Appendix E, SymbolsDefines the symbols used on the CyAn analyzer labels.ConventionsThis manual uses the following conventions: Bold font indicates instrument manual titles. Bold indicates a screen icon. Italics font indicates screen text displayed by the instrument.IMPORTANT IMPORTANT is used for comments that add value to the step or procedure being performed.Following the advice in the IMPORTANT message adds benefit to the performance of a piece of equipmentor to a process.NOTE NOTE is used to call attention to notable information that should be followed during installation, use,or servicing of this equipment.-xxiv0000050K

CHAPTER 1InstallationGeneral Laboratory InformationIMPORTANT Your Beckman Coulter Representative is responsible for uncrating, installing, and initial setupof the CyAn ADP. Contact your Beckman Coulter Representative before relocating the instrument.CyAn ADP Installation SpecificationsHeating and air conditioning vents or fans are not recommended directly above the CyAn ADPbecause of the resulting temperature fluctuation, vibration, and possible dust.Tab

NOTE Because the CyAn ADP has been designed to operat e as a Class 1 Laser Device, the instrument must be operated with all light containment tubes in place and all protective light seals intact. Most laser components of the CyAn ADP flow cytometer are Class IIIB to Class IV.