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1IntroductionOptical SystemOptical SystemThe optical system of the detector is shown in Figure below. Its illuminationsource is a combination of a deuterium-arc-discharge lamp for the ultraviolet(UV) wavelength range and a tungsten lamp for the visible (VIS) andshort-wave near-infrared (SWNIR) wavelength range. The image of thefilament of the tungsten lamp is focused on the discharge aperture of thedeuterium lamp by means of a special rear-access lamp design which allowsboth light sources to be optically combined and share a common axis to thesource lens. The achromat (source lens) forms a single, focused beam of lightthrough the flow cell. Each cell room and lamp are separated by a quartzwindow which can be cleaned or replaced. In the spectrograph, light is beingdispersed onto the diode array by a holographic grating. This allowssimultaneous access to all wavelength information.&HOO VXSSRUW ZLQGRZ7XQJVWHQ ODPS&RXSOLQJ OHQV'HXWHULXP ODPS FKURPDW VRXUFH OHQV ROPLXP R[LGH ILOWHU)ORZ FHOO6SHFWUR OHQV6OLW*UDWLQJ'LRGH DUUD\Figure 1Optical System of the DetectorAgilent 1260 Infinity DAD and MWD User Manual11

1IntroductionOptical SystemLampsAchromat(Source Lens)The light source for the UV-wavelength range is a deuterium lamp with ashine-through aperture. As a result of plasma discharge in low-pressuredeuterium gas, the lamp emits light over the 190 nm to approximately 800 nmwavelength range. The light source for the visible and SWNIR wavelengthrange is a low noise tungsten lamp. This lamp emits light over the wavelengthrange 470 – 950 nm.The achromat receives the light from both lamps and focuses it so that thebeam passes through the flow cell.Holmium OxideFilterThe holmium oxide filter is electromechanically actuated. During the holmiumfilter test it moves into the light path.Cell SupportWindowThe cell support window assembly separates the holmium filter area from theflow cell area.Flow CellCompartmentThe optical unit has a flow cell compartment for easy access to flow cells. Avariety of optional flow cells can be inserted using the same quick, simplemounting system. The flow cell can be removed to check the optical andelectronic performance of the detector without having influences from theflow cell.SpectrographThe spectrograph material is ceramic to reduce thermal effects to a minimum.The spectrograph consists of the spectrograph lens, the variable entrance slit,the grating and the photodiode array with front-end electronics. Thespectrograph lens refocuses the light beam after it has passed through the flowcell. The sampling interval of the diode array is 1 nm over the wavelengthrange 190 – 950 nm. Depending on the wavelength this varies from 1.0 to 1.25diodes per nanometer (for example a diode every 0.8 to 1 nm).For a small wavelength range, the small non-linearity could be neglected. Withthe wavelength range from 190 – 950 nm a new approach is required toachieve wavelength accuracy over the full range. Each spectrograph iscalibrated individually. The calibration data is stored in the spectrograph onan EEPROM. Based on these data, the built-in processors calculate absorbancedata with linear intervals (1.0, 2.0, ) between data points. This results in anexcellent wavelength accuracy and instrument-to-instrument reproducibility.Variable EntranceSlit System12The micro-slit system makes use of the mechanical properties of siliconcombined with the precise structuring capabilities of bulk micro-machining. Itcombines the required optical functions — slit and shutter — in a simple andcompact component. The slit width is directly controlled by themicro-processor of the instrument and can be set as method parameter.Agilent 1260 Infinity DAD and MWD User Manual

IntroductionOptical System1GratingThe combination of dispersion and spectral imaging is accomplished by usinga concave holographic grating. The grating separates the light beam into all itscomponent wavelengths and reflects the light onto the photodiode array.Diode ArrayThe diode array is a series of 1024 individual photodiodes and control circuitslocated on a ceramic carrier. With a wavelength range from 190 – 950 nm thesampling interval is 1 nm.Agilent 1260 Infinity DAD and MWD User Manual13

1IntroductionSystem OverviewSystem OverviewLeak and Waste HandlingThe 1200 Infinity Series has been designed for safe leak and waste handling. Itis important that all security concepts are understood and instructions arecarefully followed. % & Figure 214Leak and waste handling concept (overview - typical stack configuration asan example)Agilent 1260 Infinity DAD and MWD User Manual

IntroductionSystem Overview1The solvent cabinet (1) is designed to store a maximum volume of 6 L solvent.The maximum volume for an individual bottle stored in the solvent cabinetshould not exceed 2.5 L. For details, see the usage guideline for the Agilent1200 Infinity Series Solvent Cabinets (a printed copy of the guideline has beenshipped with the solvent cabinet, electronic copies are available on theInternet).The leak pan (2) (individually designed in each module) guides solvents to thefront of the module. The concept covers also leakages on internal parts (e.g.the detector’s flow cell). The leak sensor in the leak pan stops the runningsystem as soon as the leak detection level is reached.The leak pan's outlet port (3, A) guides excessive overfill from one module tothe next, as the solvent flows into the next module’s leak funnel (3, B) and theconnected corrugated waste tube (3, C). The corrugated waste tube guides thesolvent to the next lower positioned module’s leak tray and sensor.The waste tube of the sampler’s needle wash port (4) guides solvents to waste.The condense drain outlet of the autosampler cooler (5) guides condensate towaste.The waste tube of the purge valve (6) guides solvents to waste.The waste tube connected to the leak pan outlet on each of the bottominstruments (7) guides the solvent to a suitable waste container.Agilent 1260 Infinity DAD and MWD User Manual15

1IntroductionBio-inert MaterialsBio-inert MaterialsFor the Agilent 1260 Infinity Bio-inert LC system, Agilent Technologies useshighest quality materials in the flow path (also referred to as wetted parts),which are widely accepted by life scientists, as they are known for optimuminertness to biological samples and ensure best compatibility with commonsamples and solvents over a wide pH range. Explicitly, the complete flow pathis free of stainless steel and free of other alloys containing metals such as iron,nickel, cobalt, chromium, molybdenum or copper, which can interfere withbiological samples. The flow downstream of the sample introduction containsno metals whatsoever.Table 1Bio-inert materials used in Agilent 1260 Infinity SystemsModuleMaterialsAgilent 1260 Infinity Bio-inert Quaternary Pump(G5611A)Titanium, gold, platinum-iridium, ceramic,ruby, PTFE, PEEKAgilent 1260 Infinity Bio-inert High-Performance Autosampler(G5667A)Upstream of sample introduction: Titanium, gold, PTFE, PEEK, ceramicDownstream of sample introduction: PEEK, ceramicAgilent 1260 Infinity Bio-inert Manual Injector(G5628A)PEEK, ceramicAgilent 1260 Infinity Bio-inert Analytical Fraction Collector(G5664A)PEEK, ceramic, PTFEBio-inert Flow Cells:Standard flow cell bio-inert, 10 mm, 13 µL, 120 bar ( 12 MPa) for MWD/DAD,includes Capillary Kit Flow Cells BIO (p/n G5615-68755) (G5615-60022)(for Agilent 1260 Infinity Diode Array Detectors DAD G1315C/D)PEEK, ceramic, sapphire, PTFEMax-Light Cartridge Cell Bio-inert ( 10 mm, V(s) 1.0 µL) (G5615-60018) andMax-Light Cartridge Cell Bio-inert ( 60 mm, V(s) 4.0 µL) (G5615-60017)(for Agilent 1200 Infinity Series Diode Array Detectors DAD G4212A/B)PEEK, fused silicaBio-inert flow cell, 8 µL, 20 bar (pH 1–12) includes Capillary Kit Flow Cells BIO(p/n G5615-68755) (G5615-60005)(for Agilent 1260 Infinity Fluorescence Detector FLD G1321B)PEEK, fused silica, PTFE16Agilent 1260 Infinity DAD and MWD User Manual

IntroductionBio-inert MaterialsTable 11Bio-inert materials used in Agilent 1260 Infinity SystemsModuleMaterialsBio-inert heat-exchanger G5616-60050(for Agilent 1290 Infinity Thermostatted Column Compartment G1316C)PEEK (steel-cladded)Bio-inert Valve headsG4235A, G5631A, G5639A: PEEK, ceramic(Al2O3 based)Bio-inert Connection capillariesUpstream of sample introduction: TitaniumDownstream of sample introduction: Agilent uses stainless-steel-claddedPEEK capillaries, which keep the flowpath free of steel and providepressure stability to more than 600bar.NOTETo ensure optimum bio-compatibility of your Agilent 1260 Infinity Bio-inert LC system, donot include non-inert standard modules or parts to the flow path. Do not use any parts thatare not labeled as Agilent “Bio-inert”. For solvent compatibility of these materials, see“Material Information” on page 93.Agilent 1260 Infinity DAD and MWD User Manual17

118IntroductionBio-inert MaterialsAgilent 1260 Infinity DAD and MWD User Manual

Agilent 1260 Infinity DAD and MWD User Manual2Site Requirements and SpecificationsSite Requirements20Physical Specifications23Performance SpecificationsSpecifications 24Specification Conditions2436This chapter provides information on environmental requirements, physicaland performance specifications.Agilent Technologies19

2Site Requirements and SpecificationsSite RequirementsSite RequirementsA suitable environment is important to ensure optimal performance of theinstrument.Power ConsiderationsThe module power supply has wide ranging capability. It accepts any linevoltage in the range described in Table 2 on page 23. Consequently there is novoltage selector in the rear of the module. There are also no externallyaccessible fuses, because automatic electronic fuses are implemented in thepower supply.WA R N I N GHazard of electrical shock or damage of your instrumentationcan result, if the devices are connected to a line voltage higher than specified. Connect your instrument to the specified line voltage only.WA R N I N GThe module is partially energized when switched off, as long as the power cord isplugged in.Repair work at the module can lead to personal injuries, e.g. electrical shock, whenthe cover is opened and the module is connected to power. Always unplug the power cable before opening the cover. Do not connect the power cable to the instrument while the covers are removed.C AU T I O NInaccessible power plug.In case of emergency it must be possible to disconnect the instrument from the powerline at any time. Make sure the power connector of the instrument can be easily reached andunplugged. Provide sufficient space behind the power socket of the instrument to unplug thecable.20Agilent 1260 Infinity DAD and MWD User Manual

2Site Requirements and SpecificationsSite RequirementsPower CordsDifferent power cords are offered as options with the module. The female endof all power cords is identical. It plugs into the power-input socket at the rear.The male end of each power cord is different and designed to match the wallsocket of a particular country or region.WA R N I N GAbsence of ground connection or use of unspecified power cordThe absence of ground connection or the use of unspecified power cord can lead toelectric shock or short circuit. Never operate your instrumentation from a power outlet that has no groundconnection. Never use a power cord other than the Agilent Technologies power cord designedfor your region.WA R N I N GUse of unsupplied cablesUsing cables not supplied by Agilent Technologies can lead to damage of theelectronic components or personal injury. Never use cables other than the ones supplied by Agilent Technologies to ensureproper functionality and compliance with safety or EMC regulations.WA R N I N GUnintended use of supplied power cordsUsing power cords for unintended purposes can lead to personal injury or damageof electronic equipment. Never use the power cords that Agilent Technologies supplies with this instrumentfor any other equipment.Agilent 1260 Infinity DAD and MWD User Manual21

2Site Requirements and SpecificationsSite RequirementsBench SpaceThe module dimensions and weight (see Table 2 on page 23) allow you to placethe module on almost any desk or laboratory bench. It needs an additional2.5 cm (1.0 inches) of space on either side and approximately 8 cm(3.1 inches) in the rear for air circulation and electric connections.If the bench shall carry a complete HPLC system, make sure that the bench isdesigned to bear the weight of all modules.The module should be operated in a horizontal position.EnvironmentYour detector will work within the specifications at ambient temperatures andrelative humidity described in Table 2 on page 23.ASTM drift tests require a temperature change below 2 C/hour (3.6 F/hour)over one hour period. Our published drift specification (refer also to“Performance Specifications G1315C” on page 24) is based on theseconditions. Larger ambient temperature changes will result in larger drift.Better drift performance depends on better control of the temperaturefluctuations. To realize the highest performance, minimize the frequency andthe amplitude of the temperature changes to below 1 C/hour (1.8 F/hour).Turbulences around one minute or less can be ignored.NOTEC AU T I O NThe module is designed to operate in a typical electromagnetic environment (EN61326-1)where RF transmitters, such as mobile phones, should not be used in close proximity.Condensation within the moduleCondensation can damage the system electronics. Do not store, ship or use your module under conditions where temperaturefluctuations could cause condensation within the module. If your module was shipped in cold weather, leave it in its box and allow it to warmslowly to room temperature to avoid condensation.22Agilent 1260 Infinity DAD and MWD User Manual

2Site Requirements and SpecificationsPhysical SpecificationsPhysical SpecificationsTable 2Physical SpecificationsTypeSpecificationWeight11.5 kg (26 lbs)Dimensions(height width depth)140 x 345 x 435 mm(5.5 x 13.5 x 17 inches)Line voltage100 – 240 V , 10 %Line frequency50 or 60 Hz, 5 %Power consumption160 VA / 160 W / 546 BTUAmbient operatingtemperature0–55 C (32–131 F)Ambient non-operatingtemperature-40 – 70 C (-40 – 158 F)Humidity 95 % r.h. at 40 C (104 F)Operating altitudeUp to 3000 m (9842 ft)Non-operating altitudeUp to 4600 m (15092 ft)For storing the moduleSafety standards:IEC, EN, CSA, ULInstallation category II, Pollution degree 2For indoor use only.ISM ClassificationISM Group 1 Class BAccording to CISPR 11Agilent 1260 Infinity DAD and MWD User densing23

2Site Requirements and SpecificationsPerformance SpecificationsPerformance SpecificationsSpecificationsPerformance Specifications G1315CTable 324Performance Specifications G1315CTypeSpecificationCommentsDetection type1024-element photodiode arrayLight sourceDeuterium and tungsten lampsData rateup to 80 HzWavelength range190 – 950 nmShort term noise(ASTM) Single andMulti-Wavelength 0.7·10-5 AU at 254 and 750 nmsee "Specification Conditions"belowDrift 0.9·10-3 AU/h at 254 nmsee "Specification Conditions"belowLinear absorbancerange 2 AU (5 %) at 265 nmsee "Specification Conditions"belowWavelengthaccuracy 1 nmSelf-calibration with deuteriumlines, verification with holmiumoxide filterWavelengthbunching1 – 400 nmProgrammable in steps of 1 nmSlit width1, 2, 4 , 8, 16 nmProgrammable slitDiode width 1 nmThe UV-lamp is equipped withRFID tag that holds lamp typicalinformation.Agilent 1260 Infinity DAD and MWD User Manual

2Site Requirements and SpecificationsPerformance SpecificationsTable 3Performance Specifications G1315CTypeSpecificationCommentsFlow cellsStandard: 13 µL volume, 10 mm cellpath length and 120 bar (1740 psi)pressure maximumStandard bio-inert: 13 µL volume,10 mm cell path length and 120 bar(1740 psi) pressure maximumSemi-micro: 5 µL volume, 6 mm cellpath length and 120 bar (1740 psi)pressure maximumMicro: 2 µL volume, 3 mm cell pathlength, 120 bar (1740 psi) pressuremaximumSemi-nano: 500 nL volume, 10 mmcell path length and 50 bar (725 psi)pressure maximumNano: 80 nL volume, 6 mm cell pathlength and 50 bar (725 psi) pressuremaximumHigh pressure: 1.7 µL volume, 6 mmcell path length and 400 bar(5800 psi) pressure maximumPrep SST: 3 mm cell path length and120 bar (1740 psi) pressure maximumPrep Quartz:0.3 mm cell path lengthand 20 bar (290 psi) pressuremaximumPrep Quartz: 0.06 mm cell path lengthand 20 bar (290 psi) pressuremaximumAll flow cells are equipped withRFID tags that hold cell typicalinformation.pH range 1.0—9.5 (12.5 solventdependent with bio-inert version)Time programmableWavelength, polarity, peak width,lamp bandwidth, autobalance,wavelength range, threshold, spectrastorage modeSpectral toolsData analysis software for spectraevaluation, including spectrallibraries and peak purity functionsAgilent 1260 Infinity DAD and MWD User Manual25

2Site Requirements and SpecificationsPerformance SpecificationsTable 3Performance Specifications G1315CTypeSpecificationCommentsControl and dataevaluationAgilent ChemStation for LC (32-bit)For 1260 systems: Revision B.04.02 DSP2 oraboveFor 1100/1200 systems: Revision B.01.03 or aboveLocal ControlAgilent Instant Pilot (G4208A)For 1260 systems: B.02.11 or aboveFor other systems: B.02.09 or above26Analog outputsRecorder/integrator: 100 mV or 1 V,output range 0.001 – 2 AU, twooutputsCommunicationsController-area network (CAN),RS-232C, APG Remote: ready, start,stop and shut-down signals, LANSafety andmaintenanceExtensive diagnostics, error detectionand display (through control moduleand ChemStation), leak detection,safe leak handling, leak output signalfor shutdown of pumping system.Low voltages in major maintenanceareas.GLP featuresRFID for electronics records of flowcell and UV lamp conditions (pathlength, volume, product number,serial number, test passed, usage)Early maintenance feedback (EMF)for continuous tracking of instrumentusage in terms of lamp burn timewith user-setable limits and feedbackmessages. Electronic records ofmaintenance and errors. Verificationof wavelength accuracy with built-inholmium oxide filter.HousingAll materials recyclable.OthersElectronic temperature control (ETC)for the complete optical unitAgilent 1260 Infinity DAD and MWD User Manual

Site Requirements and SpecificationsPerformance Specifications2Performance Specifications G1315DTable 4Performance Specifications G1315DTypeSpecificationCommentsDetection type1024-element photodiode arrayLight sourceDeuterium and tungsten lampsData rateup to 20 HzWavelength range190 – 950 nmShort term noise(ASTM) Single andMulti-Wavelength 0.7·10-5 AU at 254 and 750 nmsee "Specification Conditions"belowDrift 0.9·10-3 AU/h at 254 nmsee "Specification Conditions"belowLinear absorbancerange 2 AU (5 %) at 265 nmsee "Specification Conditions"belowThe UV-lamp is equipped withRFID tag that holds lamp typicalinformation.Wavelength accuracy 1 nmSelf-calibration with deuteriumlines, verification with holmiumoxide filterWavelength bunching 1 – 400 nmProgrammable in steps of 1 nmSlit width1, 2, 4 , 8, 16 nmProgrammable slitDiode width 1 nmAgilent 1260 Infinity DAD and MWD User Manual27

2Site Requirements and SpecificationsPerformance SpecificationsTable 4Performance Specifications G1315DTypeSpecificationCommentsFlow cellsStandard: 13 µL volume, 10 mm cellpath length and 120 bar (1740 psi)pressure maximumStandard bio-inert: 13 µL volume,10 mm cell path length and 120 bar(1740 psi) pressure maximumSemi-micro: 5 µL volume, 6 mm cellpath length and 120 bar (1740 psi)pressure maximumMicro: 2 µL volume,

Self-test 144 Filter Test 145 Intensity Test 148 Holmium Oxide Test 151 ASTM Drift and Noise Test 154 Cell Test 156 Using the Built-in Test Chromatogram 158 Wavelength Verification and Calibration 160 9 Maintenance 163 Introduction to Maintenance 164 Cautions and Warnings 165 .