Transcription
EVOS fl FLUOROPHORE LISTDyeOther CubesGFP light cubeRFP light cubeTexas Red light cubeCy5 light cubeCFP light cubeDAPI light cubeDAPI light cubeDAPI light cubeDAPI light cubeDAPI light cubeDAPI light cubeYFP light cubewww.amgmicro.comEM Peak357/44 EX447/60 xcellentAcridine362462ExcellentAlexa Fluor 350346442ExcellentAlexa Fluor xcellentBFP382448ExcellentCalcein Blue373440GoodCalcein Violet401451ExcellentCalcofluor White350440ExcellentCascade iFMU (pH 9.0)357450ExcellentER-Tracker Blue-White DPX371554ExcellentFast Blue360440ExcellentFluospheres Blue356412ExcellentHoechst 33258345487ExcellentHoechst 33342347483ExcellentLive/Dead Fixable Blue Dead Cell Stain344442ExcellentLysoSensor Blue374424ExcellentLysoTracker Blue373422ExcellentMarina lorobimane380461GoodPacific Blue405455ExcellentPBFI360420GoodPhorwite AR360430GoodQuinine Sulfate349461ExcellentsgBFP (Super Glow BFP)387450GoodSITS365460GoodSpectrumBlue 405449ExcellentThioflavin TCN350460ExcellentThiolyte378483GoodTrue Blue365425ExcellentUvitex SFC365435ExcellentY66H360442ExcellentDAPI light cubeDAPI light cube(click to view)ABS PeakSpectrum Match*EVOS LED Light Cube Compatibility REV 2Published 20 JUL 2009
EVOS fl FLUOROPHORE LISTDyeGFP light cube(click to view)DAPI light cubeRFP light cubeTexas Red light cubeCy5 light cubeCFP light cubeGFP light cubeOther CubesGFP light cubeGFP light cubeGFP light cubeGFP light cubeGFP light cubeGFP light cubeYFP light cubewww.amgmicro.comABS PeakEM Peak470/22 EX525/50 EMSpectrum Match*5-FAM (carboxyfluorescein)492518GoodAcridine Orange500526GoodAcridine Yellow470550ExcellentAlexa Fluor 430 432540GoodAlexa Fluor 480 490510ExcellentAlexa Fluor 488 500540GoodAmplex Gold512540GoodATTO hine470540ExcellentBCECF499540GoodBODIPY 492/515490540GoodBODIPY 505/515502540GoodBodipy cium Green-1506540FairCatskill Green 540482540ExcellentCellTracker 492540ExcellentcyQUANT 89540ExcellentDiversa erald494518GoodFluoro-Jade B480525ExcellentFluor-X494520GoodFluoSpheres Yellow-Green501513GoodGFP (EGFP)488507ExcellentHCS LipidTox Green498506FairHPTS (Solvent Green 7)455510ExcellentLive/Dead Fixable Green498526GoodLucifer Yellow423543FairLysoSensor Green448503ExcellentEVOS LED Light Cube Compatibility REV 2Published 20 JUL 2009
EVOS fl FLUOROPHORE LISTDye(click to view)DAPI light cubeRFP light cubeTexas Red light cubeCy5 light cubeCFP light cubeGFP light cubeOther CubesGFP light cubeGFP light cubeGFP light cubeGFP light cubeGFP light cubeGFP light cubeYFP light cubewww.amgmicro.comABS PeakEM PeakSpectrum Match*LysoTracker Green504511GoodMagnesium Green506530FairmHoneydew478561ExcellentMitoTracker Green FM490512ExcellentNBD Amine450530ExcellentNBD-X467538ExcellentNeurotrace 500/525 entOregon Green 488498524GoodOregon Green 514512532FairPicoGreen502522GoodQdot 525UV525FairQdot 545UV543FairRhodamine 110496520GoodRhodamine 123507529FairRhodamine Green497523GoodRhodol Green496523GoodrsGFP (red shifted GFP, 511GoodsgGFP (Super Glow GFP)474509ExcellentSodium Green506532GoodSpectrumGreen 498522GoodSurf Green EX469526ExcellentSYBR Gold495439GoodSYBR Green I497521GoodSYBR Safe507524GoodSYTO 11506525FairSYTO 13487505GoodSYTO 16490520ExcellentSYTO 45452484GoodSYTO 9482500ExcellentSYTO RNASelect503527GoodSYTOX -1513531FairVibrant Dyecycle Green505532GoodwtGFP (wild type GFP, non-UV 507ExcellentYOYO-1491509ExcellentEVOS LED Light Cube Compatibility REV 2Published 20 JUL 2009
EVOS fl FLUOROPHORE LISTOther Cubes(click to view)DAPI light cubeGFP light cubeTexas Red light cubeCy5 light cubeCFP light cubeRFP light cubeDyeRFP light cubeRFP light cubeRFP light cubeRFP light cubeRFP light cubeRFP light cubeYFP light cubewww.amgmicro.comABS PeakEM PeakSpectrum Match*RFP light cube531/40 EX593/40 EMAlexa Fluor 532532554GoodAlexa Fluor 546561572GoodAlexa Fluor 555555565ExcellentAlexa Fluor 568578603GoodAlexa Fluor 594590621GoodAmplex Red571583ExcellentAmplex UltraRed568583ExcellentBOBO-3570602GoodBodipy R6G527547GoodBodipy TMR544569ExcellentBodipy TRX588620FairCalcium Orange549575ExcellentCarboxy SNARF-1548587ExcellentCarboxyrhodamine 6G524547GoodCellMask Orange556571ExcellentCellTrace Calcein Red-Orange575591GoodCellTracker Orange547575ExcellentCellTracker 51568ExcellentDsRed 79GooddTomato554581GoodEosin524545GoodEthidium Bromide521602GoodEthidium Homodimer-1530618ExcellentFluospheres Orange539560GoodFluospheres Red575607GoodFocalCheck Double Orange540555GoodFocalCheck Double Red589612GoodHCS LipidTox e/Dead Fixable Red593613FairLOLO-1566580GoodLysotracker Red577592ExcellentMitoTracker Orange551576ExcellentMitoTracker Red578599GoodEVOS LED Light Cube Compatibility REV 2Published 20 JUL 2009
EVOS fl FLUOROPHORE LISTDyeOther CubesDAPI light cubeGFP light cubeTexas Red light cubeCy5 light cubeCFP light cubeRFP light cubeRFP light cubeRFP light cubeRFP light cubeRFP light cubeYFP light cubeRFP light cube(click to view)www.amgmicro.comABS PeakEM PeakSpectrum rine568585GoodNile ntPhycoerythrin xcellentPropidium Iodide530618GoodPro-Q 551576ExcellentRhodamine Phalloidin557576ExcellentRhodamine ellentSpectrumOrange 559588ExcellentSulfarhodamine 101578593GoodSYPRO Ruby468633FairSYTO 11505525FairSYTO RNASelect504526FairSYTOX AAdvanced Dead Cell Stain546645FairSYTOX Orange567570ExcellentTag rTetramethylrhodamine (TRITC)555580ExcellentTexas boRFP553574ExcellentVibrant Dyecycle Green504532FairVibrant Dyecycle Orange518562ExcellentX-Rhod-1570576GoodEVOS LED Light Cube Compatibility REV 2Published 20 JUL 2009
DyeOther CubesDAPI light cubeGFP light cubeRFP light cubeCy5 light cubeCFP light cubeTexas Red light cubeTexas Red light cubeTexas Red light cubeTexas Red light cubeYFP light cubeTexas Red light cube(click to view)www.amgmicro.comABS PeakEM PeakSpectrum Match*Texas Red light cube585/29 EX624/40 llent7-AAD549644GoodalamarBlue570584FairAlexa Fluor 568578603GoodAlexa Fluor 594590618ExcellentAlexa Fluor 610610629FairAmplex Red570583FairAmplex Ultra Red567582FairBOBO-3571606GoodBodipy 630/650-X625641FairBodipy TR-X588621ExcellentCalcium Crimson589608ExcellentCellTrace BODIPY TR methyl esther597625ExcellentCellTrace calcein red-orange575592GoodCellTracker Red CMTPX584613ExcellentCy 3.5581597GoodDsRed2563588FairFluospheres Crimson microspheres620646GoodFluospheres Red microspheres577608ExcellentHCS CellMask Red cytoplasmic/nuclear621644FairHCS CellMask Red stain587614ExcellentHCS LipidTOX Red582615ExcellentHCS NuclearMask Red624644FairHC Red594649FairKatushka (Turbo FP635)588635ExcellentLiveDead Fixable Red Dead Cell stain595613ExcellentLOLO-1568580FairLysoTracker Red573592GoodMagic Red570630GoodMitroTracker 5ExcellentmPlum590649FairmRaspberry598625GoodNile Red 3608Excellentresorufin570583Fairrhodamine phalloidin558577Fairrhodamine Red-X572591GoodEVOS LED Light Cube Compatibility REV 2Published 20 JUL 2009
EVOS fl FLUOROPHORE LISTDye(click to view)DAPI light cubeGFP light cubeRFP light cubeCFP light cubeYFP light cubeTexas Red light cubeOther CubesABS PeakEM PeakSpectrum Match*sulforhodamine 101577593GoodSYTO 17620635GoodSYTO 59622645FairSYTO 61619649FairSYTOX AADvanced dead cell stain546647GoodTexas 12628GoodYO-YO-3612631GoodABS PeakEM PeakSpectrum Match*Cy5 light cube628/40 EX692/40 EMCy5 light cubeCy5 light cubeCy5 light cubeCy5 light cubeDyewww.amgmicro.comAlexa Fluor 594591621FairAlexa Fluor 610611630FairAlexa Fluor 633631650GoodAlexa Fluor 635634647FairAlexa Fluor 647 hydrazide650670GoodAlexa Fluor 660 antibody conjugate663691ExcellentAlexa Fluor 680 antibody conjugate679704GoodAlexa Fluor 680 R phycoerythrin568700FairAlexa Fluor 680 allophycocyanin680703ExcellentAPC (Allophycocyanin)651660GoodBodipy 630/650X626641FairBodipy cellentCellMask Deep Red plasma membrane 658GoodDiD es crimson microspheres621646GoodFluoSpheres dark red microspheres657683ExcellentFocalCheck DoubleFarRed General stain669692GoodFocalCheck DoubleFarRed Ring stain655677ExcellentFxCycle Far Red stain641657GoodHCS CellMask Deep Red stain649670ExcellentHCS CellMask Red cytoplasmic/nuclear stain621642GoodHCS LipidTOX Deep Red634652GoodHCS NuclearMask Deep Red635687ExcellentEVOS LED Light Cube Compatibility REV 2Published 20 JUL 2009
EVOS fl FLUOROPHORE LISTOther CubesDyeDAPI light cubeGFP light cubeRFP light cubeTexas Red light cubeCy5 light cube(click to view)ABS PeakEM PeakSpectrum Match*HCS NuclearMask Red622644GoodMitoTracker Deep Red -60649681ExcellentSYTO-62650681ExcellentSYTOX S PeakEM PeakSpectrum Match*442/46 EX510/42 EM434477ExcellentABS PeakEM PeakSpectrum Match*500/24 EX542/27 EM514527CFPDyeCFP light cubeECFPDyeYFPYFP light cubeEYFPExcellent* Predicts (but does not guarantee) imaging performance with the designated EVOS light cube.For dyes that are not on this list, please contact AMG for assistance.www.amgmicro.comEVOS LED Light Cube Compatibility REV 2Published 20 JUL 2009
Live/Dead Fixable Green 498 526 Good Lucifer Yellow 423 543 Fair LysoSensor Green 448 503 Excellent Other Cubes (click to view) DAPI light cube RFP light cube Texas Red light cube Cy5 light cube CFP light cube YFP light cube .
