C101-E168AUV-VIS SpectrophotometerUV-1900i

Navigate Your WayEasy to Operate, Obtain Answers Easily and RapidlyEasy-to-use user interface designErgonomic touch-screen displayHigh Performance to Meet Diverse NeedsThe instrument is equipped with an ultra high-speed scan, which can acquire aspectrum in a few seconds, with the lowest level of stray light and noise in its classAdvanced Regulatory ComplianceValidation functions enable checks in accordance with Pharmacopeia (JP, USP, and EP)to be performed easilyIn combination with LabSolutions DB/CS, comply with FDA 21 CFR Part 11 andPIC/S GMP guidelinesUV-i SelectionUV-1900iUV-2600i/2700iUV-3600i PlusSolidSpec -3700i

Easy to Operate, Obtain Answers Easily and RapidlyThe instrument is equipped with astylus pen, allowing operation bythis pen or a finger.Easy-to-Use InterfaceGrasp the Current Status andOperating Procedures at a GlanceThe UV-1900i on-screen user interface includes large, easy-to-seeicons deployed on a black background, so the instrumentsettings are evident at a glance. In addition, the large,easy-to-see icons improve intuitive understanding, whichenables users to quickly become familiar with the operations.Furthermore, the user interface is designed to minimizetransitions between windows, so users do not get confusedduring the operations.4Display languages are available in eight languages (Japanese, English,Chinese, Spanish, Portuguese, German, French, Russian).

Ergonomic Hardware DesignThe control panel is ergonomically designed and positionedat the optimum viewing angle for the user. Users canoperate easily with any posture. Since the panel ispressure-sensitive, the device can be operated with glovedfingers or a stylus pen. The stylus pen can be stored rightnext to the control panel.Navigation TabsImprove UsabilityIn quantitation mode on the UV-1900i,the stages of the entire measurementprocess and the current status are alwaysshown on the display. As a result, usersknow immediately what to do in the nextstep.UV-1900iUV-VIS Spectrophotometer5

High Performance to Meet Diverse NeedsLow Stray LightStray light is at 0.5 % max. (198 nm), making accurate measurements are possible up tothe vicinity of 2 Abs even in the ultraviolet region. In addition, high-concentrationsamples can be quantified accurately.The figure on the right is a calibration curve for acetic acid, created with absorbance at200 nm.The correlation coefficient is 0.9997 and correct measured values are obtained even inthe vicinity of 2 Abs. Linearity will be lost in the high absorbance region due to thestray light.6Abs.Calibration Curve3.002.50Instrument with 1.00 % Stray LightUV-1900i2.001.501.000.500. (mg/L)3.00

Ultra-Fast ScanSpectra can be acquired as fast as 29,000 nm/min. Ultra-fast scan is effective in tracking chemical reactions in a short time. Inaddition to the absorbance change at specified wavelengths, spectra can also be acquired in a short time with the UV-1900i.Therefore, more detailed behavior can be investigated by observing spectra with the UV-1900i.The figures below show the analysis of the particle agglomeration process when salts are added to silver nanoparticles.Measurements of the 300 to 700 nm region were performed in ultra-fast scan mode. In addition to the decrease ofabsorbance at 400 nm and the increase of absorbance at 480 nm, the temporal changes of spectra can also be 700.00.620Absorbance at 480 nm (Abs)0.21Absorbance at 400 nm (Abs) after 0 minRed: after 0.5 minBlue: after 1 minGreen: after 2 minOrange: after 5 minPurple: after 10 min0.091002003004005006000.07Time (sec)Absorption Spectra of Silver NanoparticlesTemporal Changes of Absorbance at 400 and 480 nmHigh Reproducibility and Repeatability AccuracyThe photometric repeatability accuracy is 0.0002 Abs max. (0.5 Abs and 1.0 Abs). With this high photometric repeatability accuracy,variance in the measurement results is suppressed, enabling more accurate quantitation and the detection of low-concentrationsamples.The figure on the right is a calibration curve forcaffeine, created with absorbance at 273 nm.The calibration curve has an Abs 0.0528 Conc.The lower limit of quantitation determined fromthe standard deviation is 0.0051 mg/L.Note: One method of determining the lower limit ofquantitation is to use ten times the standard deviation.This is an actual measured value and is not guaranteed.No.12345678910StandardDeviation σAbsorbance of Blank Solution (273 00001-0.000040.000010.000050.0000252.5Calibration CurveAbs. (mg/mL)40.0UV-1900iUV-VIS Spectrophotometer7

Various Functions for Comfortable Daily MeasurementNetwork Connectivity FunctionThe UV-1900i provides network access via wireless connectivity.Data can now be transferred to a PC via a network.Thanks to wireless printing, multiple UV units can print from a single printer.(A router and other network equipment must be installed to use a network.)Data transfer function*1PictBridge function*2*1: Optional expanded memory is required. The instrument is not compatible with control via a network.*2: A PictBridge compatible printer is required.Sleep Mode and Wakeup FunctionAnalysis can start the instant the user arrives at the laboratoryin the morning. The instrument requires no time to warm up.PreviousdayStart Time9 : 00NextmorningReadyBar-Code Reader and Keyboard Entry FunctionSample names and numerical values can be entered by a bar-code reader or from the keyboard.This saves time when entering sample names for a multiple sample analysis, and prevents sample misidentification and otherhuman errors.*3*3: Use a bar-code reader and keyboard with a USB connection.8

A Diversity of Measurement ModesPhotometricSpectrumQuantitationMeasures the photometric value at asingle wavelength or multiple (up toeight) wavelengths.Measures a sample spectrum usingwavelength scanning.Generates a calibration curve from themeasurement of standards, and thencalculates the concentrations ofunknowns.KineticsTime CourseBiomethodMeasures absorbance changes as afunction of time, and obtains theenzymatic activity values. The kineticsmeasurement method or the ratemeasurement method can be selected.Measures changes over time inphotometric values at a specifiedwavelength.Quantifies DNA or proteinconcentrations.UV-1900iUV-VIS Spectrophotometer9

ApplicationsFoods1.00This is an example of the analysis of food dyes. By using0.80Absorbance (Abs.)ultra-fast scan mode, the time needed for routine spectralchecks can be shortened. The 350 nm to 900 nm region can bemeasured at 1 nm intervals in approx. 4 seconds.0.600.400.200.00350 400800900Absorption Spectra of Food DyesPharmaceuticals and Life SciencesThis is an example of the analysis of λDNA. Trace quantities (on1.2the order of a few µL) can be measured by combining theOptical path length: 1 mminstrument with Nano Stick and TrayCell .Black: 500Red: 250Blue: 100Green: 50Orange: 25Unit: ng/µL1.0Calibration Curve1.21.0Absorbance (Abs.)Absorbance (Abs.)Using TrayCell, a calibration curve for 4 µL of λDNA wasobtained in the range between 25 ng/µL and 500 ng/µL.600Wavelength (nm)0.50.5Nano Stick0.0TrayCell2200.025030033025.0 100.0300.0Concentration (ng/µL)Wavelength (nm)ChemistryThis is an example of the analysis of colored cellophane tape.Materials can be confirmed quantitatively by using LabSolutionsUV-Vis and color measurement software.0.901000.800.700.600.5060yTransmittance gth (nm)7000.200.300.40x0.500.600.70780Transmittance Spectra of Colored Cellophane Tape100.10Chromaticity Diagram of XY Color System0.80500.0

AccessoriesExpanded Memory(P/N 207-23119-41)Specifications:(P/N 204-58909)Used in transmittancemeasurement of thin samplessuch as films and filters. Itholds the samples foranalysis.Long-Path Rectangular Cell Holder(P/N 207-23510-41)Holds test tube instead ofsample compartment.The UV-1900i main unit can store up to 999 sets of data.The saved data can be read out from a PC.(A network connection is required for data readout.)Film HolderTest Tube HolderOutside diameter: φ15 to 22 mmHeight: 75 to 115 mmNote: Test tube is not included.Multi-Cell Sample Compartment(P/N 204-23118-01)Holds two rectangular cells with an opticalpath length of 10, 20, 30, 50, 70, or 100 mm.(P/N 206-69160-41)Holds up to six 10-mm squarecells on the sample side. Notemperature control capability.Number of cells: 6 on the sample side1 on the reference sideNote: Square cells are not included, please purchase separately.Sipper UnitP/NStandard Sample VolumeSipper Unit 160L (Standard Sipper)Model206-23790-512.0 mLSipper Unit 160T (Triple-Pass Sipper)206-23790-521.5 mLSipper Unit 160C (Constant-Temperature Sipper)206-23790-532.5 mLSipper Unit 160U (Supermicro Sipper)206-23790-540.5 mLFour types of sipper units with different flow cells are available. Thestepping motor-driven peristaltic pump ensures reliable and smoothaspiration of sample solution.(Direct driving is possible from the UV-1900i so no interface is required.)Note: The use of a Solenoid Valve (Fluoropolymer) (P/N 204-06599-01)and the SWA-2 Sample Waste Unit (206-23820-58) arerecommended when strong acids, strong alkalis, or organicsolvents are to be measured.CPS-100 Cell Positioner, ThermoelectricallyTemperature Controlled(P/N 206-29500-41)This attachment permits measurement of up to six sample cells underconstant-temperature conditions. Combination of this attachment andthe Kinetics mode provides measurement of temperature-sensitiveenzyme kinetics of one to six samples.Number of cells: 6 on the sample side (temperature-controlled)1 on the reference side (temperature not controlled)Temperature control range: 16 C to 60 CTemperature display accuracy (difference from the true value): 0.5 CTemperature control precision (variation of temperature): 0.1 CAmbient temperature: 15 C to 35 CNote: Square cells (P/N 200-34442) are not included, please purchaseseparately.A USB adapter CPS(P/N 206-25234-91)is required.TCC-100 Thermoelectrically TemperatureControlled Cell Holder(P/N 206-29510-41)Uses Peltier effect for controlling the sample and referencetemperature, so no thermostated bath or cooling water is required.Number of cells: One each on the sample and reference sides(temperature-controlled)Temperature control range: 7 C to 60 CTemperature display accuracy (difference from the true value): 0.5 CTemperature control precision (variation of temperature): 0.1 CNote: Square cells (P/N 200-34442) are notincluded, please purchaseseparately.UV-1900iUV-VIS Spectrophotometer11

Standard Software: LabSolutions UV-VisThe combination of the UV-1900i, offering lower stray light levels and higher accuracy,with LabSolutions UV-Vis software, which achieves labor-saving measurements,provides a more convenient analytical environment.Setting ParametersFrom Measurement to Data OutputSmooth OperabilityImproved Productivity of Data Analysis OperationsFo u r M eas u rem e n t M o d e sData analysis and data output operations can be performed atFour separate measurement modes for spectral, quantitative,photometric, time-course, and automatic measurements(optional) enable measurements to be performed using intuitiveoperations.the same time (simultaneously) as data measurement. Timespent outputting or analyzing data can also be reduced bysimultaneously sending data to an Excel spreadsheet in realtime or saving data as text. The software can also automaticallyperform post-processing of measured data, such asprocessing/correcting spectra, and perform pass/fail judgmentsof measurement results (automatic spectral evaluation).Startmeasurem en tFour Measurement Mode WindowsIns tru men t C on t ro l P a n e lare separate from the measurement window. The control panelsinclude various functionality that is laid out for superiorvisibility. Each measurement window connects seamlessly to thecorresponding parameter settings window.F inis hm ea s ur em e ntInstrument parameter settings can be specified via panels thatFi nis h m eas u r em ent sf or m u lt ip le s am ple s12Analyze data in Excel concurrently with data measurement(Excel data analysis real-time transfer function)Easily transfer data to externaldata analysis software(simultaneous text saving andmatrix output functions)

Data ManagementAutomatic Spectral Evaluation(Spectral Evaluation Function)Stronger Data ManagementIn addition to regular file management in folders on a PC, idealBy specifying various evaluation criteria for measurementsolutions for saving data in a database with sophisticatedresults, spectra judgments can be made functionality and compliance with ER/ES-relatedregulations are also available.Optional SoftwareLabSolutions DB UV-VisLabSolutions CS UV-VisD at abas e M anagem entManaging data in a database can prevent the overwriting ordeletion of analysis data. Furthermore, during postrunanalysis, the data can be managed using version numbers,so there are no concerns about overwriting the data.PostrunanalysisAnalysisVer. 1In the report creation window, reports can either be prepared basedPostrunanalysisVer. 2Ver. 3Reduces operator errors.on a previously specified report format or freely laid out based onvarious parameters, data, or other elements.UV-1900iUV-VIS Spectrophotometer13

Advanced Regulatory ComplianceFull Support for Pharmacopeia, GLP/GMP, FDA 21 CFR Part 11 and Other RegulationsInstrument Validation Functions Compliant with JP, USP, and EPThis instrument can not only run checks for nine JIS items, but also those stipulated in the Japanese Pharmacopoeia (JP), UnitedStates Pharmacopeia (USP), and the European Pharmacopoeia (EP). Naturally, the hardware is also compliant with the specificationsrequired by each Pharmacopeia. In addition, the check conditions can be saved. As a result, once the conditions are saved, checks canbe performed easily just by calling them up as needed. Check results can also be saved.Main ScreenParameters Setting ScreenCheck Results ScreenDuring Testing ScreenImproved Security FunctionsAn external control security function has been added to provide more support forcompliance with regulations. Three user authority levels, "Administrator","Developer", and "Operator", can be set for instrument users.Resolution of 1 nm, the Highest in its ClassIn addition to achieving a resolution of 1 nm, the highest in its class, by using a monochromator with a Czerny-Turner mounting, theUV-1900i also features a compact, bright optical system. The instrument is more than capable of meeting the wavelength resolutionrequired in the European Pharmacopoeia.14

Compatible with Validation from PC SoftwareValidation can be implemented with PC software by using the optionally available UV validation software.In addition to simplifying daily inspections, this makes instrument performance checks and records management easier, enabling moresecure regulatory compliance. Inspection results can not only be printed but also saved to a file, so theresults can be called up later for confirmation. The inspection parameters can also be saved to separate files forperiodic and routine inspections, and then called up for use. The user can select confirmation of instrument performance indicatorsas per JIS K0115 General rules for molecular absorptiometric analysis, aswell as the general test methods in the Japanese Pharmacopeia, USPand various EP inspections. (Order inspection jigs and reagentsseparately.)Support for FDA 21 CFR Part 11, PIC/S GMP Guidelines and Other Regulations and GuidelinesEnsuring the integrity of data (database management), including the user management, user authority management, and data audittrails required for compliance with FDA 21 CFR Part 11, PIC/S GMP guidelines, and other ER/ES regulations, is possible.LabSolutions DB UV-Vis or UVProbe / LabSolutions DB SystemThe system allows for data management and user managementwith a database. Compliant with ER/ES regulations, the system isoptimally configured for customers using a PC.LabSolutions CS UV-Vis or UVProbe / LabSolutions CS System (Network System)The system is optimally configured for customers who want to manage data on a server together with LC and GC data for ER/EScompliance.LabSolutions serverAnalysis equipmentroom or an officeClient PCAcquisitioncontroller PCUVFTIRRFICPMSGCMSEDXLCGCLCGCAATOCParticle sizeanalyzerCombine with Multi Data Registration license to enableintegrated management of these instruments’ data.UV-1900iUV-VIS Spectrophotometer15

UV-1900iUV-1900i Website spectro/uv/uv-1900/index.html-Automated support functions utilizing digital technology, such as M2M, IoT , and ArtificialIntelligence (AI), that enable higher productivity and maximum reliability.-Allows a system to monitor and diagnose itself, handle any issues during data acquisitionwithout user input, and automatically behave as if it were operated by an expert.-Supports the acquisition of high quality, reproducible data regardless of an operator’s skilllevel for both routine and demanding applications.LabSolutions and Analytical Intelligence Logo are trademarks of Shimadzu Corporation.TrayCell is a registered trademark of Hellma GmbH & Co. KG. in Germany.Excel is a registered trademark or trademark of the Microsoft Corporation in the USA and other countries.For Research Use Only. Not for use in diagnostic procedures.This publication may contain references to products that are not available in your country. Please contact us to check the availability of theseproducts in your country.Company names, products/service names and logos used in this publication are trademarks and trade names of Shimadzu Corporation, itssubsidiaries or its affiliates, whether or not they are used with trademark symbol “TM” or “ ”.Third-party trademarks and trade names may be used in this publication to refer to either the entities or their products/services, whether or notthey are used with trademark symbol “TM” or “ ”.Shimadzu disclaims any proprietary interest in trademarks and trade names other than its contents of this publication are provided to you “as is” without warranty of any kind, and are subject to change without notice. Shimadzudoes not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication. Shimadzu Corporation, 2019First Edition: November 2019, Printed in Japan 3655-01004-30AIT

Ultra-Fast Scan Spectra can be acquired as fast as 29,000 nm/min. Ultra-fast scan is effective in tracking chemical reactions in a short time. In addition to the absorbance change at specified wavelengths, spectr